Glutathione S-transferases(GSTs) are a family of isofunctional detoxifying enzymes that conjugate glutathione to a variety of electrophilic compounds in the cytoplasm of aerobic cells. The GST isoenzymes are divided into 3 classess(basic,
neutral,
and
acidic forms). Among three classes of GST isoenzymes GST-L and GST-P are basic and acidic form that were purified from the human liver and placenta, respectively.
Immunohistochemical study was done to compare the distribution pattern of GST isoenzymes in the major organs of rat and human, using polyclonal antibodies against GST-L and GST-P raised in rabbit by avidin biotin peroxidase method.
In the rate immunohintochemica(staining of GST-L showed strong positive reaction in bronchial epithelium, proximal convoluted tubules, corpus luteum, oviduct epithelium, endometrial glands, zona reticularis, parietal cells, hepatocytes, sperms,
and
astrocytes While GST-P showed moderate to strong positive reaction in proximal convoluted tubules, epidermis, hair follicles, parietal cells, bile ducts and oligodentroglia.
In the human, GST-L showed moderate to strong positive in bronchial epithelium, proximal convoluted tubules, zon a reticularis, parietal cells and hepatocytes. While GST-P showed strong positive reaction in bronchial epithelium, proximal
convouluted
tubules, corpus luteum, oviduct epithelium, endometrial glands, epidermis, hair follicles, parietal cells, hepatocytes, bile ducts, astrocytes and oligodntroglia.
In conclusion, distribution patterns of GST-P and GST-L in major organs were different in organ to organ and between rat and human, suggests that major isoenzyme of GST were different in organs and in species.
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